Home > Biological Detection > Technology > Microscopy > Scanning Electron Microscopy (SEM)

Scanning Electron Microscopy (SEM) is a powerful technique that can achieve 500,000 x magnification. SEM utilizes lenses to focus high energy electrons to create a beam that sweeps over the surface of the sample to create the image; electrons are reflected, absorbed or conducted when they interact with the sample. The sample is placed under vacuum prior to analysis. Biological samples must be coated in gold prior to analysis by SEM to preserve the integrity of the sample and prevent it from changing or decaying during the SEM process.